美国短毛黑水貂快速生长过程中转录组差异表达分析

doi:10.11843/j.issn.0366-6964.2019.09.006

畜牧兽医学报 ›› 2019, Vol. 50 ›› Issue (9): 1787-1801.doi: 10.11843/j.issn.0366-6964.2019.09.006

美国短毛黑水貂快速生长过程中转录组差异表达分析

荣敏, 张然然, 涂剑峰, 王天娇, 孙丽敏, 杨颖, 徐佳萍, 邢秀梅*

中国农业科学院特产研究所特种经济动物分子生物学重点实验室, 长春 130112

收稿日期:2019-04-03 出版日期:2019-09-23 发布日期:2019-09-23
通讯作者: 邢秀梅,主要从事特种经济动物资源保护与利用研究,E-mail:xingxiumei2004@126.com
作者简介:荣敏(1980-),女,河北景县人,博士,主要从事毛皮动物资源遗传育种与繁殖研究,E-mail:rongmin12@126.com
基金资助:
吉林省科技厅自然科学基金项目（20150101113JC）；中国农业科学院特种动物遗传资源创新团队基金

American Short-hair Black Mink Transcriptome Differential Expression Genes Analysis During the Rapid Growth Process

RONG Min, ZHANG Ranran, TU Jianfeng, WANG Tianjiao, SUN Limin, YANG Ying, XU Jiaping, XING Xiumei*

State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences of Chinese Academy of Agricultural Sciences, Changchun 130112, China

Received:2019-04-03 Online:2019-09-23 Published:2019-09-23

摘要/Abstract

摘要： 旨在对美国短毛黑水貂快速生长过程中转录组差异表达进行分析。本试验将美国短毛黑水貂45、90日龄各3只健康公貂的胸肌组织作为试验材料，利用Illumina HiSeq^TM2500高通量测序平台建立转录组文库，对两个生长阶段差异显著性的基因进行GO功能富集和KEGG Pathway分析，寻找调控水貂快速生长期的相关候选基因和代谢通路，并利用qRT-PCR对转录组测序结果进行验证。结果表明，以P<0.05，|log₂FC|>1为条件筛选到279个显著差异基因，对这些差异基因进行GO和KEGG分析，筛选到与肌肉生长相关显著富集GO条目有66条、相关差异表达基因44个，其中上调20个，下调24个；与肌肉生长相关显著富集KEGG通路12条，如p53信号通路、PPAR信号通路、FOXO信号通路等。这些差异基因可能在水貂的快速生长过程中起到调控作用，可以作为后续研究水貂生长发育的候选基因。本研究结果为探究水貂生长发育调控机制及培育大体型水貂品种奠定基础。

Abstract: This study aimed to analyze the differential expression genes based on transcriptome during the rapid growth process of American short-hair black mink. In this study, the breast muscle of 45 and 90 days old healthy American short-hair black mink (3 independent repetitions) were used as experimental material, the transcriptome library was established using Illumina HiSeq^TM2500 high-throughput sequencing platform. GO and KEGG analysis were performed on the differential expression genes between the two stages, and the candidate genes and metabolic pathways regulating the rapid growth of mink were screened. qRT-PCR was used to validate the result of transcriptome sequencing. The results showed that 279 significant differentially expressed genes were screened out with P<0.05 and|log₂FC|>1 as the criterion. GO enrichment analysis of these genes showed that 66 GO terms were significantly enriched and 44 differentially expressed genes were related to muscle growth, among which 20 were up-regulated and 24 were down-regulated. Twelve pathways related to muscle growth were screened out, such as p53, PPAR and FOXO signaling transduction pathways. These genes may play a regulatory role in the rapid growth process of mink, and can be used as candidate genes for the further study on the growth and development of mink. The results could provide a theoretical basis for exploring the regulation mechanism of growth and development in mink, and breeding large-size mink breeds.

中图分类号:

S865.22

荣敏, 张然然, 涂剑峰, 王天娇, 孙丽敏, 杨颖, 徐佳萍, 邢秀梅*. 美国短毛黑水貂快速生长过程中转录组差异表达分析[J]. 畜牧兽医学报, 2019, 50(9): 1787-1801.

RONG Min, ZHANG Ranran, TU Jianfeng, WANG Tianjiao, SUN Limin, YANG Ying, XU Jiaping, XING Xiumei*. American Short-hair Black Mink Transcriptome Differential Expression Genes Analysis During the Rapid Growth Process[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(9): 1787-1801.

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美国短毛黑水貂快速生长过程中转录组差异表达分析

American Short-hair Black Mink Transcriptome Differential Expression Genes Analysis During the Rapid Growth Process

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